Influence of Fermentation Process Parameters on L- Glutaminase production by Bacillus subtilis RSP-GLU

An L-glutaminase producing microorganism was isolated and identified as Bacillus subtilis RSP-GLU. The production pattern of the enzyme by isolated microorganism was studied in submerged fermentations (Smf). Initially various process parameters such as pH, incubation temperature, inoculum concentration and agitation speed were optimized based on the one-at-a-time method. It was noticed that at pH 7.0 is the optimum for L-glutaminase production. The maximum production of the enzyme was observed at temperature 37 0C, initial inoculum concentration 2% and agitation speed 100 rpm grown culture broth at 24 hours of incubation. At the optimized conditions0.682 g L-1 of biomass and 196 U m-1 of enzyme was obtained.